On an island game, a player can freely build settlers at first and research map making to get early triremes to scout. If this is a standard game with four players on map size one with landmass 70, or similarly size 2 with landmass 30, the player needs to devote his capital to building units while other cities produce settlers. Strategy depends on map size, map generator, land size, and starting units. A player needs to build offensive/defensive units quickly, try to control his borders, research new governments, and destroy the weakest nations. Strategy depends on the map you're playing on. You can also learn by playing with others or against the AI.Ģ) Press 'B' key or goto your menus>work>build city. It is recommended that you try playing solitaire a few times, so that you can get a feel for how things work, but this is not necessary. Now that you have Freeciv running, you'll want to play your first few games. If you're looking for ideas on playing Freeciv, then keep reading!! If you have never played the Civilization games, it's easiest to start by reading the Freeciv Manual, available at Manual. If you're looking for how to get Freeciv running, look in doc/README. If you're looking for how to install Freeciv, look in Install. The Freeciv playing HOWTO Originally by Michael Hohensee (aka Zarchon) What kinds of military units are the most useful? Should I make the game easier by increasing the starting gold? In multiplayer games, what timeout should I set? 1.2 Q: Can you give me a basic strategy?.(Load a map by typing in/load /dir/ in the input field at the bottom of thescreen, in this way it is possible to change the settings of a game.Use the map editor to change a map.)- FAIR is the most fair of all generators for the multiplayer games.It gives all users or teams identical islands.- FRACTURE generates maps from a fracture pattern. This way, nobody can whine about losing 'onaccount of that d.ned island.' - SCENARIO is used for premade maps. This means that the mountains and the hills will beplaced according to everlasting mathematical figures.- ISLAND generates islands of equal size (sometimes with some smallerislands thrown in). To fix this, our kind and lovingcoders installed the generator option.- When set to RANDOM, it creates the map using a random height generator,with islands of different (and potentially unfair) sizes.- When set to FRACTAL it generates the map using a pseudo fractal heightgenerator. Tiny Island Syndrome (TIS)is known to make people go insane. You want lots of cities,seven or eight at the least.The name of the game is to tie down as many squares of land aspossible. The first thing you want todo is build cities and explore your island. But it will get you started playing Freeciv.Part of the appeal of Freeciv is in developing new strategies.The game is divided into several stages:The Initial Expansion phaseTechnological sub-phaseThe Second Expansion phaseThe Production phaseand The Utter Annihilation of Your Enemies phaseInitial Expansion:This phase is the most critical. You can also learn by playing with others or againstthe AI.Q: Can you give me a basic strategy?First of all, this isn't a.perfect. It is recommended that you try playing solitaire afew times, so that you can get a feel for how things work, but this isnot necessary. The Freeciv playing HOWTOOriginally by Michael Hohensee (aka Zarchon)If you're looking for how to install Freeciv, look in INSTALL.If you're looking for how to get Freeciv running, look in README.If you have never played the Civilization games, it's easiest tostart by reading the Freeciv Manual, available separately at:you're looking for ideas on playing Freeciv, then keep reading!!Now that you have Freeciv running, you'll want to play yourfirst few games.
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In 2020, he completed a Master’s in Professional Studies program in Design Management and Communications at Georgetown University. He is a huge proponent of lifelong learning, and in recent years has completed professional certificates in project management and budget and finance. in microbiology from New York University. in Biology and a minor in Applied Economics and Management. McKinney graduated from Cornell University with a B.S. His administrative portfolio includes a range of offices with functionalities that span the student training experience, including career strategy, proposal development for institutional training programs, student and postdoctoral engagement, and recruitment and marketing.ĭr. He is also a member of the Board of Directors and serves as Treasurer of the National Postdoctoral Association. 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If set to `chr` the mapping position (binned to 10Gb intervals) will be appended to `UB` (ideal for InDrops+dropEst). u, -umi-extension TEXT In case UMI is too short to guarantee uniqueness (without information from the ampping) set this parameter to `chr`, `Gene` ro `bp` U, -without-umi If this flag is used the data is assumed UMI-less and reads are counted instead of molecules (default: off) l, -logic TEXT The logic to use for the filtering (default: Default) Important: cells reads should not be distributed over multiple bamfiles is not supported!! c, -onefilepercell If this flag is used every bamfile passed is interpreted as an independent cell, otherwise multiple files are interpreted as batch of different cells to be analyzed together. s, -metadatatable FILE Table containing metadata of the various samples (csv formatted, rows are samples and cols are entries) e, -sampleid PATH The sample name that will be used to retrieve informations from metadatatable o, -outputfolder PATH Output folder, if it does not exist it will be created. If -bcfile is not specified all the cell barcodes will be included.Ĭell barcodes should be specified in the bcfile as the `CB` tag for each read b, -bcfile FILE Valid barcodes file, to filter the bam. Runs the velocity analysis outputting a loom file įor example if we want to run the pipeline on the cellranger output folder mypath/sample01. If p is prepended a more complete ( but huge ) pickle report is printed ( default : 0 ) - v, - verbose Set the vebosity level : - v ( only warinings ) - vv ( warinings and info ) - vvv ( warinings, info and debug ) - help Show this message and exit. gtf file containing intervals to mask - l, - logic TEXT The logic to use for the filtering ( default : Default ) - M, - multimap Consider not unique mappings ( not reccomended ), - samtools - threads INTEGER The number of threads to use to sort the bam by cellID file using samtools - samtools - memory INTEGER The number of MB used for every thread by samtools to sort the bam file - t, - dtype TEXT The dtype of the loom file layers - if more than 6000 molecules / reads per gene per cell are expected set uint32 to avoid truncation ( default run_10x : uint16 ) - d, - dump TEXT For debugging purposes only : it will dump a molecular mapping report to hdf5. Usage : velocyto run10x SAMPLEFOLDER GTFFILE Runs the velocity analysis for a Chromium 10 X Sample 10 XSAMPLEFOLDER specifies the cellranger sample folder GTFFILE genome annotation file Options : - s, - metadatatable FILE Table containing metadata of the various samples ( csv fortmated rows are samples and cols are entries ) - m, - mask FILE. If you are interested in running velocyto with only one technique you can directly jump to that section without loss of information (this also means that some of the information will be repeated). We will now describe the use of the technique specific subcommands. Please regard these subcommands as the recommended and easiest way to run velocyto, especially if you are unsure of all the options to pass to velocyto run.įor more flexibility and advanced usage, velocyto run should be used directly.įurthermore, to adapt velocyto to custom/new techniques the user may want to consider modification of the counting pipeline, this does not require deep rewrite of the internals but just the creation of a new logic, for more information consult the section about the Logic interface API. They take care of passing the appropriate options for each technology furthermore they performa a minimal check the inputs provided make sense and some of them infer the path of some of the input files. These subcommands are just wrappers of the main command velocyto run. The currently available are: run10x, run_smartseq2, run_dropest However, for some of the most commonly used scRNA-seq chemistries, we provide a set of ready-to-use subcommands. 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